VIDEO PROTOCOLS: From Washing to Mounting is the series which guides you through the essential steps in microscopy sample preparation procedure. From initial washing to final mounting. Let’s get started!
Mounting is a critical step in microscopy preparation procedures, aimed at preserving the sample and enhancing the image quality during observation. Proper mounting maintains the structural integrity of your specimen, reduces image distortions (like spherical…
DAPI (4′,6-diamidino-2-phenylindole) is a fluorescent stain that binds strongly to DNA. It is commonly used in fluorescence microscopy for visualizing cell nuclei because its fluorescence is enhanced when bound to double-strand DNA. The protocol: For staining…
The purpose of secondary antibody staining is to bind to the primary antibody, thus amplifying the signal and allowing the spatial location of the primary antibody to be visualized through the emission of specific fluorophore signals in…
The purpose of primary antibody staining is to bind a specific antigen present in your cell or tissue sample. This is a key step in immunostaining protocols, which are widely used to visualize specific proteins or other…
Blocking is a crucial step in fluorescence microscopy to prevent nonspecific binding of antibodies and reduce the occurrence of artifacts. Protocol Typically, either a 3% solution of Bovine Serum Albumin (BSA), Normal Goat Serum (NGS), or…
The procedure for transferring the cover glass from the petri dish to a drop of PBS on a Parafilm surface involves several careful steps. Protocol First, secure a piece of Parafilm on your bench surface using…
Triton X-100 permeabilization is a commonly employed procedure in cell biology, particularly when the goal is to enable larger molecules, such as antibodies or dyes, to penetrate the cell. This process involves treating the cells with…
Paraformaldehyde (PFA) fixation is a fundamental procedure in cell biology that involves treating cells with a solution of PFA. The reagent crosslinks proteins and thus preserves the cellular structure at a particular moment in time. The…
Before proceeding with cell fixation, it’s necessary to thoroughly wash the cells. Cell washing step is crucial for removing cell debris and other contaminants, ensuring a clean sample prior to fixation. This cell washing protocol outlines…