The mounting medium is the ENVIRONMENTfor your sample. It determines how long and how well your specimen will shine, how pure the signal will be, and the quality of the outcome.
Mounting spacer is a PROTECTIONfor your specimen. The spacer not only and easy seals and secures the sample perpendicular position to objective, it is essential protection for all fragile biological structures.
The cover glass is a WINDOWof your sample. Through the cover glass you observe the specimen. The quality, thickness, size – those are crucial propertis of it and directly influence your results.
The slide is a CARRIERfor your sample. It is not so much crucial for fluorescence, more important for transmitted light. Anyway, it could be precise and nice, with useful features and high suitability.
If you have any question regarding ADVI products in light microscopy: