This video protocol guides you through the process of using AD-SEAL mounting spacers to finalize your microscopic samples. The use of mounting spacers offers several advantages for your microscopic sample preparation. They provide a secure and stable means to attach the cover glass, ensuring that your sample stays in place during imaging. This eliminates the […]
Mounting spacers – How to easy use Read More »
Innovative sample preparation using alcohol dehydration and high refractive index medium enables acquisition of two-channel super-resolution 3D STED image of an entire oocyte Michaela Frolikova, Michaela Blazikova, Martin Capek, Helena Chmelova, Jan Valecka, Veronika Kolackova, Eliska Valaskova, Martin Gregor, Katerina Komrskova, Ondrej Horvath, Ivan Novotny https://doi.org/10.1111/jmi.13363 ABSTRACT Super-resolution (SR) microscopy is a cutting-edge method that
ADVI Mounting media and Spacers in breakthrough STED imaging Read More »
ADVI is an official partner for the EMBO Practical Course on SUPER-RESOLUTION IN LIGHT MICROSCOPY, which took place from June 18th to June 23rd, 2023. In addition to our partnership, ADVI was responsible for supplying mounting media and spacers for the preparation of microscopic samples for super-resolution microscopy techniques ranging from confocal microscopy to structured
ADVI: Official Partner for EMBO Practical Course on Super-Resolution Read More »
Mounting spacers: ANTI – FLATTENING Mounting media with microscopic spacers Cell flattening during slide preparation isn’t solely due to hardening mounting media. The capillary pressure between the microscope slide and the cover glass also contributes to this effect. High viscosity mounting medium penetrates biological structures slower and cover glass + slide initiate capillary pressure. This
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Mounting is a critical step in microscopy preparation procedures, aimed at preserving the sample and enhancing the image quality during observation. Proper mounting maintains the structural integrity of your specimen, reduces image distortions (like spherical aberration) caused by refractive index mismatch, and allows long-term storage. The protocol The protocol outlined below describes a common mounting
09 Mounting procedure Read More »
DAPI (4′,6-diamidino-2-phenylindole) is a fluorescent stain that binds strongly to DNA. It is commonly used in fluorescence microscopy for visualizing cell nuclei because its fluorescence is enhanced when bound to double-strand DNA. The protocol: For staining of nuclear DNA in common adherent cells, a DAPI concentration in the range of 1-10 µg/mL is usually sufficient.
Secondary antibody staining of the primary antibody. Amplifies the signal and allowing the spatial location of the antibody to be visualized.
07 Secondary antibody staining Read More »
Primary antibody staining is a key step in microscopy immunostaining protocols for specific proteins or other molecules visualization.
06 Primary antibody staining Read More »
Blocking is a crucial step in fluorescence microscopy to prevent nonspecific binding of antibodies and reduce the occurrence of artifacts.
The procedure for transferring the cover glass from the petri dish to a drop of PBS on a Parafilm surface involves several careful steps.
04 From Petri dish to Parafilm Read More »
