Triton X-100 permeabilization is a commonly employed procedure in cell biology, particularly when the goal is to enable larger molecules, such as antibodies or dyes, to penetrate the cell. This process involves treating the cells with a solution of Triton X-100, a detergent that disrupts the lipid bilayers of cell membranes without completely destroying them, thereby increasing the membrane’s permeability.
Protocol
Typically, a solution of 0.2% Triton X-100 is sufficient for most permeabilization procedures. After applying the Triton X-100 solution, it’s standard to allow it to function for around 5 minutes at room temperature. This permeabilization step is crucial for many assays as it allows for the intracellular staining and investigation of cellular components. This protocol outlines the BASIC procedure. However, many procedures are often modified to suit a particular purpose.
