AD-MOUNT H + DAPI

AD-MOUNT H+DAPI – Hardening Mounting Medium with Nuclear Stain

AD-MOUNT H+DAPI is a hardening fluorescence mounting medium with integrated DAPI (4′,6-diamidino-2-phenylindole) for direct nuclear DNA staining. It offers high structural preservation, long-term photostability, and prevents dye diffusion—making it ideal for archiving cellular and tissue samples for fluorescence imaging.

This medium forms a cured protective layer that secures the sample and supports delicate structures, including membrane- and cytoskeleton-associated features. AD-MOUNT H+DAPI is fully compatible with common fluorophores such as FITC, Cy3, Alexa Fluor® dyes, mCherry, and phalloidin-conjugates. It is particularly suited for widefield and confocal microscopy where both DNA visualization and durability are needed.

⚠ May cause flattening of cells due to curing—common in hardening media.

Key Properties

• Integrated DAPI – Provides rapid, stable nuclear counterstaining.

• Hardening Protection – Prevents dehydration, drift, and dye loss.

• Fluorophore Versatility – Compatible with FITC, Cy3, Alexa Fluor® dyes, phalloidin.

• Structure-Preserving – Maintains morphology of sensitive specimens.

• Refractive Index: 1.45

• Storage: +4 °C; stable for up to 12 months after opening

• Format: Liquid (hardening), no mixing required

• Research Use Only

📄 Download technical datasheet: TDS_ADMOUNT-H_ADM-017